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MTOX1071 Sigma

Kidney PTEC MDR1 KnockoutCells [MDR1 knock out (-/-)]

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Properties

Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Kidney Drug Transporter/Toxicity Models
biological source   renal proximal convoluted tubule epithelial cells from human female
OMIM accession no.   171050
storage temp.   −196°C
Gene Information   human ... ABCB1(5243)

Description

Application

See technical bulletin for detailed protocols

Other Notes

ADME/Tox cell lines license. These products are covered by the “Consumable Vial” License as described in Exhibit 1.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Features and Benefits

Sigma′s human RPTEC control SA7K clone cells and knockout (KO) cells exhibit normal renal proximal tubule characteristics, functional renal uptake and efflux transporters and are sensitive to human nephrotoxicants making them ideal for studying drug-transporter interactions and early detection of renal toxicants. The SA7K clone should be used as a control for Sigma′s human RPTEC KO cells
• Sigma′s human RPTEC MDR1 (P-gp) Knockout (KO) allows investigations of drug transporter interactions involving P-gp in the kidney.

General description

Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma′s RPTEC′s exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and α-GST. Sigma′s RPTEC′s maintain typical PT functions including albumin reabsorption, γ-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Related Products

Technical Service:

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