P1860 Sigma

Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

DOWNLOAD MSDS (PDF)

Description

Components

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Application

After 48 hours in medium, add fresh inhibitors in fresh medium.

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Price and Availability

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