|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, 4.6.x.x P-O bonds, 4.x.x.x Lyases, Biochemicals and Reagents,|
|foreign activity||Phopholipase C (lecithinase) ≤1 units/mg protein|
|Sphingomyelinase ≤40 units/mg protein|
|Gene Information||Bacillus cereus E33L ... BCZK3513(3026815)|
Acetylcholinesterase is measured according to Ellman, et al.
Used for the release of GPI anchored proteins from the membrane.
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Contains phosphate buffer salts, EDTA and stabilizer
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≥200 units/mg protein
Type I, lyophilized powder, 10-50 units/mg protein
Type XIV, lyophilized powder, ≥150 units/mg protein
buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)
Certificate of Analysis
Glycosylphosphatidylinisotol (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting they a...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Cancer, Glycosylations, Transduction
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
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The complement regulatory proteins CD55 (decay accelerating factor) and CD59 are expressed on the inner acrosomal membrane of human spermatozoa as well as CD46 (membrane cofactor protein). Cummerson JA, Flanagan BF, Spiller DG, et al. Immunology 118(3), 333-42, (2006)
Contrasting effects of phosphatidylinositol- and phosphatidylcholine-specific phospholipase C on apoptosis in cultured endothelial cells. Liu X, Zhao Q, Araki S, et al. Endothelium 13(3), 205-11, (2006)
Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. Shakuntala I, Malik SV, Barbuddhe SB, et al. Vet. Microbiol. 117(2-4), 229-34, (2006)
Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, receptor-like protein tyrosine phosphatase beta, is required for intoxication in AZ-521 Cells. Nakayama M, Hisatsune J, Yamasaki E, et al. Infect. Immun. 74(12), 6571-80, (2006)
Apolipoprotein CIII-induced THP-1 cell adhesion to endothelial cells involves pertussis toxin-sensitive G protein- and protein kinase C alpha-mediated nuclear factor-kappaB activation. Kawakami A, Aikawa M, Nitta N, et al. Arterioscler. Thromb. Vasc. Biol. 27(1), 219-25, (2007)
Involvement of phosphatidylinositol-phospholipase C in immune response to Salmonella lipopolysacharide in chicken macrophage cells (HD11). He H, Genovese KJ, Nisbet DJ, et al. Int. Immunopharmacol. 6(12), 1780-7, (2006)
Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits. Cao Z, Zhang J, Li Y, et al. Protein Expr. Purif. 52(2), 306-12, (2007)
Over-expression of the truncated ghrelin receptor polypeptide attenuates the constitutive activation of phosphatidylinositol-specific phospholipase C by ghrelin receptors but has no effect on ghrelin-stimulated extracellular signal-regulated kinase 1/2 activity. Chu KM, Chow KB, Leung PK, et al. Int. J. Biochem. Cell Biol. 39(4), 752-64, (2007)
Roles of brain phosphatidylinositol-specific phospholipase C and diacylglycerol lipase in centrally administered histamine-induced adrenomedullary outflow in rats. Shimizu T, Yamaguchi N, Okada S, et al. Eur. J. Pharmacol. 571(2-3), 138-44, (2007)
Membrane-associated phosphoinositides-specific phospholipase C forms from Catharanthus roseus transformed roots. Echevarría-Machado I, Martínez-Estévez M, Muñoz-Sánchez JA, et al. Mol. Biotechnol. 35(3), 297-309, (2007)
Treatment of mouse oocytes with PI-PLC releases 70-kDa (pI 5) and 35- to 45-kDa (pI 5.5) protein clusters from the egg surface and inhibits sperm-oolemma binding and fusion. Coonrod, S.A. Dev. Biol. 207, 334, (1999)
Enzymatic properties of phosphatidylinositol inositolphosphohydrolase from Bacillus cereus. Substrate dilution in detergent-phospholipid micelles and bilayer vesicles. Sundler, R., et al. J. Biol. Chem. 253, 4175, (1978)
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