P6534 Sigma

Phospholipase A2 from porcine pancreas

ammonium sulfate suspension, ≥600 units/mg protein

DOWNLOAD MSDS (PDF)

Synonym: Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase

Description

Analysis Note

Protein determined by biuret.

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 at 37 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 5.5

Price and Availability

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