Synonym: Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,|
|form||ammonium sulfate suspension|
|Gene Information||pig ... PLA2G1B(445525)|
Protein determined by biuret.
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
One unit will hydrolyze 1.0 μmole of soybean
Suspension in 3.2 M (NH4)2SO4 solution, pH 5.5
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Phospholipases. Dennis, E.A., Boyer, P.D., ed. The Enzymes New York , (1983) 16, 307
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|D6518||Diethylenetriaminepentaacetic acid, ≥99% (titration)|
|P9375||1,10-Phenanthroline monohydrate, reagent grade|
|ED2SS||Ethylenediaminetetraacetic acid disodium salt dihydrate, reagent grade, 98.5-101.5% (titration)|
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