Sal I from Streptomyces albus G
buffered aqueous glycerol solution
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CAS Number 81295-38-7
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MDL number MFCD00165804
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
| Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Core Bioreagents, |
| form | buffered aqueous glycerol solution |
| concentration | 10,000 units/mL |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Specificity
Recognition sequence: 5′-G/TCGAC-3′
Cutting results: a 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Physical form
Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 50% glycerol (v/v), 10 mM dithioerythritol, at 4 °C
Application
SalI is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.
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