Cfo I from Clostridium formicoaceticum
buffered aqueous glycerol solution
DOWNLOAD MSDS (PDF)-
CAS Number 81811-52-1
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MDL number MFCD00166595
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Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
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Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, |
| form | buffered aqueous glycerol solution |
| concentration | 10,000 units/mL |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SL (B3782).
Linkage
Isoschizomer: Hha I
Specificity
Recognition Sequence: 5′-GCG/C-3′
Cutting results: a 2-10-fold Cfo I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Not complete at 65 °C for 10 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol, 0.1mM PMSF, 50% glycerol (v/v), at 4 °C
Application
CfoI is restriction endonuclease used in molecular biology methods to cleave DNA at the recognition site 5′-GCG/C-3′ to generate restriction fragments with 3′- cohesive ends.
Price and Availability
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buffered aqueous glycerol solution
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