|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,|
|form||buffered aqueous glycerol solution|
|shipped in||wet ice|
Supplied with 10x Restriction Endonuclease Buffer SA (B7531).
Comment: Sac I activity is inhibited by salt concentrations above 10 mM.
Recognition sequence: 5′-GAGCT/C-3′
Ligation and recutting results: After 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation : Inactivated at 65 °C for 15 minutes.
Solution in 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
SacI is a restriction enzyme used in molecular biology applications to cleave DNA at the recognition site 5′-GAGCT/C-3′, generating DNA restriction fragments with 3′-cohesive ends.
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