|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,|
|form||buffered aqueous glycerol solution|
|shipped in||wet ice|
Supplied with 10x Restriction Endonuclease Buffer SA (B7531).
One unit of Acc I cleaves approximately 98% of the substrate DNA (pBR322 DNA).
Recognition Sequence: 5′-GT/(A,C)(G,T)AC-3′
Ligation and recutting results: After 2-10-fold Acc I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity retained after 10 minutes at 65 °C. Five-fold enhanced activity occurs at 55°C. Enzyme inactivated at 80 °C for 20 minutes.
Solution in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 10 mM 2-mecarptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v), at 4°C
AccI is a restriction endonuclease that is used in molecular biological methods to cleave the recognition sequence 5′-GT/(A,C)(G,T)AC-3′, generating DNA fragments with 5′-cohesive termini.
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