Acc I from Acinetobacter calcoaceticus buffered aqueous glycerol solution

Properties

Related Categories	3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, Molecular Biology, Molecular Biology Enzymes, Molecular Biology Tested, Restriction Endonucleases, Restriction Enzymes More...
form	buffered aqueous glycerol solution
concentration	≥5,000 units/mL
shipped in	wet ice
storage temp.	−20°C

Description

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SA (B7531).

One unit of Acc I cleaves approximately 98% of the substrate DNA (pBR322 DNA).

Specificity

Recognition Sequence: 5′-GT/(A,C)(G,T)AC-3′
Ligation and recutting results: After 2-10-fold Acc I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity retained after 10 minutes at 65 °C. Five-fold enhanced activity occurs at 55°C. Enzyme inactivated at 80 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 10 mM 2-mecarptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v), at 4°C

Application

AccI is a restriction endonuclease that is used in molecular biological methods to cleave the recognition sequence 5′-GT/(A,C)(G,T)AC-3′, generating DNA fragments with 5′-cohesive termini.