|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index,|
|form||buffered aqueous glycerol solution|
|shipped in||wet ice|
Supplied with 10x Restriction Endonuclease Buffer SM (B3158).
Recognition sequence: 5′-GCATG/C-3′
Ligation and recutting results: After 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Solution in 20 mM Tris-HCl , pH 8.2, 1 mM EDTA, 350 mM NaCl, 7 mM 2-mercaptoethanol, 0.1 mM PMSF, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), at 4 °C
Characterization of a site-specific restriction endonuclease Sph l from Streptomyces phaeochromogenes. Fuchs, L.Y., et al. Gene 10, 391, (1980)
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