Xba I from Xanthomonas badrii
buffered aqueous glycerol solution
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CAS Number 81295-42-3
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MDL number MFCD00679266
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Popular Documents: Datasheet (PDF)
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Properties
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Enzyme Class Index, |
| form | buffered aqueous glycerol solution |
| concentration | 10,000 units/mL |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Comment: Xba I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains). May cleave at secondary recognition sites under non-optimal conditions.
Specificity
Recognition sequence: 5′-T/CTAGA-3′
Ligation and recutting results: After 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 100 mM NaCl, 0.1 mM PMSF, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.02% polydocanol at 4 °C
Application
XbaI is a restriction enxyme that cleaves DNA at the recognition site 5′-T/CTAGA-3′ to generate DNA fragments with 5′-cohesive termini.
Price and Availability
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