|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate and PTM Analysis,|
|secondary activity||≥100,000 units/mL β-glucuronidase (at pH 5.0)|
|shipped in||wet ice|
Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. 2 Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate. 3
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).
Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates. 1
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Type HP-2, aqueous solution, ≥100,000 units/mL
Type H-2, aqueous solution, ≥85,000 units/mL
Type VIII, lyophilized powder, 20-40 units/mg solid
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Type H-1, sulfatase ≥10,000 units/g solid
2. SCOPE This procedure applies to all products that have a specification for Sulfatase activity, except Sulfatase from Aerobacter aerogenes, Sigma-Aldrich Product Number S1629.
Keywords: Extinction coefficient
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2. Chemoenzymatic synthesis of diverse thiohydroximates from glucosinolate-utilizing enzymes from Helix pomatia and Caldicellulosiruptor saccharolyticus Kopycki Biotechnol. Lett. 33(5), 1039-46, (2011)
Supplementation of difructose anhydride III enhanced elevation of plasma equol concentrations and lowered plasma total cholesterol in isoflavone-fed rats. Tamura, A., et al. Br. J. Pharmacol. 96, 442-9, (2006)
A canine Arylsulfatase G (ARSG) mutation leading to a sulfatase deficiency is associated with neuronal ceroid lipofuscinosis. Abitbol M, Thibaud JL, Olby NJ, et al. Proc. Natl. Acad. Sci. U. S. A. 107(33), 14775-80, (2010)
Detection of zearalenone and its metabolites in naturally contaminated porcine follicular fluid by using liquid chromatography-tandem mass spectrometry. Sambuu R, Takagi M, Shiga S, et al. J. Reprod. Dev. 57(2), 303-6, (2011)
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Impact of PAH on biological health parameters of soils of an Indian refinery and adjoining agricultural area--a case study. Chaudhary P, Singh SB, Chaudhry S, et al. Environ. Monit. Assess. 184(2), 1145-56, (2012)
Fy(a)/Fy(b) antigen polymorphism in human erythrocyte Duffy antigen affects susceptibility to Plasmodium vivax malaria. King CL, Adams JH, Xianli J, et al. Proc. Natl. Acad. Sci. U. S. A. 108(50), 20113-8, (2011)
Human O-sulfated metabolites of (-)-epicatechin and methyl-(-)-epicatechin are poor substrates for commercial aryl-sulfatases: implications for studies concerned with quantifying epicatechin bioavailability. Saha S, Hollands W, Needs PW, et al. Pharmacol. Res. 65(6), 592-602, (2012)
Proton-in-flight mechanism for the spontaneous hydrolysis of N-methyl O-phenyl sulfamate: implications for the design of steroid sulfatase inhibitors. Edwards DR and Wolfenden R J. Org. Chem. 77(9), 4450-3, (2012)
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Arylsulfatase G inactivation causes loss of heparan sulfate 3-O-sulfatase activity and mucopolysaccharidosis in mice. Kowalewski B, Lamanna WC, Lawrence R, et al. Proc. Natl. Acad. Sci. U. S. A. 109(26), 10310-5, (2012)
Gene expression profiling identifies ARSD as a new marker of disease progression and the sphingolipid metabolism as a potential novel metabolism in chronic lymphocytic leukemia. Trojani A, Di Camillo B, Tedeschi A, et al. Cancer Biomark. 11(1), 15-28, (2011)
Compartmentalization and regulation of arylsulfatase activities in Streptomyces sp., Microbacterium sp. and Rhodococcus sp. soil isolates in response to inorganic sulfate limitation. Cregut M, Piutti S, Slezack-Deschaumes S, et al. Microbiol. Res. 168(1), 12-21, (2013)
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Effects of the halophytes Tecticornia indica and Suaeda fruticosa on soil enzyme activities in a Mediterranean Sabkha. Ouni Y, Lakhdar A, Rabhi M, et al. Int. J. Phytoremediation 15(2), 188-97, (2013)
Pharmacokinetics of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol and conjugate metabolites in healthy human subjects. Zick SM, Djuric Z, Ruffin MT, et al. Cancer Epidemiol. Biomarkers Prev. 17(8), 1930-6, (2008)
The gene controlling the indole glucosinolate modifier1 quantitative trait locus alters indole glucosinolate structures and aphid resistance in Arabidopsis. Pfalz M, Vogel H, and Kroymann J Plant Cell 21(3), 985-99, (2009)
The pharmacokinetic behavior of the soy isoflavone metabolite S-(-)equol and its diastereoisomer R-(+)equol in healthy adults determined by using stable-isotope-labeled tracers. Kenneth DR Setchell et al Am. J. Clin. Nutr. 90, 1029-37, (2009)
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