MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA
Targets no known mammalian genes
DOWNLOAD MSDS (PDF)Synonym: MISSION® Control Vectors
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MDL number MFCD07785395
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Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Cell Biology, Functional Genomics and RNAi, General Stem Cell Biology, MISSION Controls in DNA Format, MISSION shRNA, |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
General description
This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.
The MISSION Non-Target shRNA Control Vector is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target human and mouse genes, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
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