MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA
Targets no known genes from any species
DOWNLOAD MSDS (PDF)Synonym: MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA control, non-target shRNA, shRNA control
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MDL number MFCD07785395
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Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA, MISSION shRNA Controls |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
General description
The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Price and Availability
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