|Related Categories||Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, Embryonic Stem Cell Biology Growth Factors,|
|recombinant||expressed in E. coli|
|mol wt||mol wt 23.9 kDa|
|impurities||<0.1 EU/μg endotoxin, tested|
|suitability||cell culture tested|
|Swiss-Prot accession no.||P13725|
|shipped in||wet ice|
|Gene Information||human ... OSM(5008)|
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi’s sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. Recombinant human Oncostatin M is a 23.9 kDa protein, containing 209 amino acid residues.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Lyophilized from 10 mM Sodium Citrate, pH 4.0.
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi’s sarcoma cells, OSH can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. The human OSM gene encodes for a 252 amino acid polypeptide, containing 25 amino acid signal sequence for secretion and a 227 precursor protein. Proteolytic processing of this precursor removes an 18 amino acid C-terminal peptide and generates the mature OSM form. Recombinant human Oncostatin M is a 23.9 kDa protein, containing 209 amino acid residues.
Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Rose, T.M., et al. Proc. Natl. Acad. Sci. U. S. A. 88, 8641-8645, (1991)
Bruce, A.G., et al. Cytokine Reference, 585-598, (2001)
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