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T3913 Sigma

Tris-Borate-EDTA buffer

5× concentrate, powder blend

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Synonym: TBE buffer

Tris-Borate-EDTA buffer 5× concentrate, powder blend
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Properties

Related Categories Biological Buffers, Buffer Convenience Packaging, Buffers A to Z, Molecular Biology, Nucleic Acid Electrophoresis,
form   powder blend
impurities   DNase, RNase and NICKase, none detected
suitability   suitable for gel electrophoresis (after dilution to working concentration)

Description

Application

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.1, TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.2 TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Price and Availability

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