Synonym: Factor IIa
|Related Categories||3.4.x.x Peptidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,|
|Gene Information||cow ... F2(280685)|
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Lyophilized powder containing sucrose, sodium chloride and Tris.
Lyophilized from saline sodium citrate buffer, pH 6.5
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the treatment of inherited platelet disorders. 1
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lyophilized powder, 40-300 NIH units/mg protein (biuret)
lyophilized powder, 600-2,000 NIH units/mg protein (biuret)
powder, cell culture tested, ≥1,500 NIH units/mg protein (E
lyophilized powder, ≥2,000 NIH units/mg protein (E
Certificate of Analysis
Certificate of Origin
The search for a functional thrombin receptor, using expression cloning methods, led to the discovery of a G protein-coupled receptor that mediates the actions of thrombin on platelets and endothelia...
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
Integrin AlphaIIbβ3 Activation In Chinese Hamster Ovary Cells And Platelets Increases Clustering Rather Than Affinity TA., B., et al. J. Bacteriol. 285, 1841-1849, (2010)
RNA-seq analysis of transcriptomes in thrombin-treated and control human pulmonary microvascular endothelial cells. Cheranova D, Gibson M, Chaudhary S, et al. J. Vis. Exp. (72), doi:10.3791/4393, (2013)
Cholesterol enrichment of rabbit platelets enhances the Ca(2+) entry pathway induced by platelet-derived secondary feedback agonists. Shiraishi M, Tamura K, Egoshi M, et al. Life Sci. 92(14-16), 838-44, (2013)
Characterization of platelet aminophospholipid externalization reveals fatty acids as molecular determinants that regulate coagulation. Clark SR, Thomas CP, Hammond VJ, et al. Proc. Natl. Acad. Sci. U. S. A. 110(15), 5875-80, (2013)
Subdomains N2N3 of fibronectin binding protein A mediate Staphylococcus aureus biofilm formation and adherence to fibrinogen using distinct mechanisms. Geoghegan JA, Monk IR, O'Gara JP, et al. J. Bacteriol. 195(11), 2675-83, (2013)
Letter by Lin et al regarding article, "Dabigatran and thrombin modulate electrophysiological characteristics of pulmonary vein and left atrium" by Chang et al. Lin GM, Li YH, and Han CL Circ. Arrhythm. Electrophysiol. 6(2), e22, (2013)
Response to letter regarding article, "Dabigatran and thrombin modulate electrophysiological characteristics of pulmonary vein and left atrium" by Chang et al. Chang CJ, Chen YC, Kao YH, et al. Circ. Arrhythm. Electrophysiol. 6(2), e23, (2013)
Controlling integrin specificity and stem cell differentiation in 2D and 3D environments through regulation of fibronectin domain stability. Martino MM, Mochizuki M, Rothenfluh DA, et al. Biomaterials 30(6), 1089-97, (2009)
Active-site mapping of bovine and human blood coagulation serine proteases using synthetic peptide 4-nitroanilide and thio ester substrates. Cho K, Tanaka T, Cook RR, et al. Biochemistry 23(4), 644-50, (1984)
Oral IIa and Xa inhibitors for prevention of stroke in atrial fibrillation: clinical studies and regulatory considerations. Deftereos S, Tsounis D, Giannopoulos G, et al. Curr. Clin. Pharmacol. 7(3), 166-74, (2012)
Binding of alpha-thrombin to surface-anchored platelet glycoprotein Ib(alpha) sulfotyrosines through a two-site mechanism involving exosite I. Zarpellon A, Celikel R, Roberts JR, et al. Proc. Natl. Acad. Sci. U. S. A. 108(21), 8628-33, (2011)
The C-terminal fragment of axon guidance molecule Slit3 binds heparin and neutralizes heparin's anticoagulant activity. Condac E, Strachan H, Gutierrez-Sanchez G, et al. Glycobiology 22(9), 1183-92, (2012)
Structure and haemostatic effects of generic versions of enoxaparin available for clinical use in Brazil: similarity to the original drug. Glauser BF, Vairo BC, Oliveira SN, et al. Thromb. Haemost. 107(2), 302-14, (2012)
A common standard is inappropriate for determining the potency of ultra low molecular weight heparins such as semuloparin and bemiparin. Jeske WP, Hoppensteadt D, Gray A, et al. Thromb. Res. 128(4), 361-7, (2011)
R. Biggs, ed. Human Blood Coagulation, Haemostasis and Thrombosis 2nd ed., Philadelphia , (1976), 722
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|L2023||Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial|
|A6191||Antipain dihydrochloride from microbial source, protease inhibitor|
|H7380||Hirudin from leeches, lyophilized powder, 300-1,500 ATU/mg protein (ATU = antithrombin units)|
|H7016||Hirudin from leeches, lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)|
Antithrombin III from bovine plasma, lyophilized powder, 200-400 units/mg protein (E
|P7626||Phenylmethanesulfonyl fluoride, ≥98.5% (GC)|
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|S9009||Sar-Pro-Arg p-nitroanilide dihydrochloride, thrombin substrate|
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