Thrombin from bovine plasma lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

Properties

Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, Blood, Cell Biology, Cell Signaling and Neuroscience, Clotting Factors, Coagulation Proteins and Reagents, Core Bioreagents, Enzyme Class Index, Enzymes, Enzymes, Inhibitors, and Substrates, Fusion Tag and Protease Removal, Immune Cell Signaling and Blood, Plasma & Blood Proteins, Plasma, Blood, and Related Proteins and Reagents, Proteases, Proteases & Protein Sequencing, Proteases for Recombinant Protein Fusion Tag Cleavage, Proteins and Derivatives, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Proteomics, Purification and Detection, Recombinant Protein Expression and Analysis, Research Essentials, Selective Proteolytic Enzymes, Serum Proteins and Related Enzymes, Serum Related Enzymes, Thrombin, Thrombins More...
form	lyophilized powder
storage temp.	−20°C
Gene Information	cow ... F2(280685)

Description

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Lyophilized powder containing sucrose, sodium chloride and Tris.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to investigate the treatment of inherited platelet disorders. ¹

Price and Availability

Related Papers

1. Treatment of inherited platelet disorders Seligsohn, U. Haemophilia 18, 161-5, (2012)

Documents

Certificate of Analysis

Certificate of Origin

Enzyme Explorer

Proteinase-Activated Receptors (172 KB )

Proteinase-Activated Receptors (89 KB )

Safety Information

Symbol	GHS07, GHS08
Signal word	Danger
Hazard statements	H315-H319-H334-H335
Precautionary statements	P261-P305 + P351 + P338-P342 + P311
Hazard Codes (Europe)	Xn
Risk Statements (Europe)	36/37/38-42
Safety Statements (Europe)	22-24-26-36/37
WGK Germany	2
RTECS	XO8950000

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Papers

Set your institution to view full text papers.

1. Treatment of inherited platelet disorders Seligsohn, U. Haemophilia 18, 161-5, (2012)

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Development Of A Drug Screening Platform Based On Engineered Heart Tissue. Hansen, A., et al. Circ. Res. 107, 35-44, (2010)

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Integrin AlphaIIbβ3 Activation In Chinese Hamster Ovary Cells And Platelets Increases Clustering Rather Than Affinity TA., B., et al. J. Bacteriol. 285, 1841-1849, (2010)

R. Biggs, ed. Human Blood Coagulation, Haemostasis and Thrombosis 2nd ed., Philadelphia , (1976), 722

Merck 14,9383

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Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)