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Articles
Cell Viability and Proliferation
Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper choice of an ass...
Mark Frei
BioFiles v6 n5, 17–21
Keywords: Cell proliferation, Flow cytometry, Mutagens, Reductions, Solvents
Protocols
Cell Quantification
For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation
Cryopreservation of Cell Lines
The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions
Resuscitation of Frozen Cell Lines
Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE
Subculture of Adherent Cell Lines
Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion
Subculture of Semi-Adherent Cell Lines
Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture
Subculture of Suspension Cell Lines
In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors
Papers
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Determination Of High-affinity Choline Uptake (HACU) And Choline Acetyltransferase (ChAT) Activity In The Same Population Of Cultured Cells. Ray, B., et al. Brain Res. 1297, 160-8, (2009)
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Porcine skin-derived stem cells can serve as donor cells for nuclear transfer. Yanhong Hao et al Cloning Stem Cells 11, 101-10, (2009)
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Cryptococcus neoformans mediator protein Ssn8 negatively regulates diverse physiological processes and is required for virulence. Lin-Ing Wang et al PLoS ONE 6, e19162, (2011)
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Development of Taenia pisiformis in golden hamster (Mesocricetus auratus). Toral-Bastida E Parasit. Vectors 4, 147, (2011)
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Human dental pulp stem cells hook into biocoral scaffold forming an engineered biocomplex. Mangano C PLoS ONE 6(4), e18721, (2011)
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Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation. Fritz JM Mol. Cancer 10, 76, (2011)
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Dendritic cells pulsed with keyhole limpet hemocyanin and cryopreserved maintain anti-tumor activity in a murine melanoma model. Teitz-Tennenbaum S Clin. Immunol. 129(3), 482-91, (2008)
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