Synonym: TAE buffer
|Related Categories||Biological Buffers, Buffer Convenience Packaging, Buffers A to Z, Molecular Biology, Nucleic Acid Electrophoresis,|
|grade||for molecular biology|
|impurities||DNase and RNase, none detected|
|suitability||suitable for electrophoresis|
|suitable for gel electrophoresis (after dilution to working concentration)|
Ready for use in gel electrophoresis after dilution to working concentrations.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaged in plastic bottles large enough to contain the concentrate after reconstitution.
Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.
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Certificate of Analysis
Certificate of Origin
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26|
Nancy-520 is a fluorescent stain for double stranded DNA (dsDNA) on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is...
BioFiles 2009, 4.1, 4.
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Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, , (1989) 23, 6.7
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