Trizma® Pre-set crystals
pH 7.0, average Mw 154.8
DOWNLOAD MSDS (PDF)Synonym: Tris crystals
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Properties
| Related Categories | BioReagent Buffers, Biological Buffers, Buffer Convenience Packaging, Buffers A to Z, Good’s Buffers, |
| grade | Biotechnology Performance Certified |
| mol wt | average Mw 154.8 |
| impurities | DNase, RNase, Protease, NICKase, none detected |
| endotoxin, tested | |
| total aerobic microbial count, tested | |
| ≤0.05 A290 | |
| ≤0.5% water (Karl Fischer) |
Description
Application
Pre-mixed combinations of TRIZMA Base and TRIZMA HCl to provide the most common pH values for Tris buffers. Dissolve in water and use, no pH adjustment is necessary. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. Guaranteed accuracy: ±0.1 pH units. If a different concentration is desired, note the concentration effect: a 0.5 M solution will have a pH 0.05 units higher; a 0.005 M solution will have a pH 0.05 units lower. If the buffer is in a pre-weighed foil pouch, dissolve one packet in 900 mL of water; add additional water to 1 liter to make a 100 mM solution. Temperature: 25 °C.
Other Notes
Certain pH electrodes do not give accurate results with Tris buffers.
Packaging
In convenient foil pouches
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Price and Availability
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