|Related Categories||BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffers A to Z,|
|Biotechnology Performance Certified|
|mol wt||average Mw 154.8|
|impurities||DNase, RNase, Protease, NICKase, none detected|
|total aerobic microbial count, tested|
|≤0.5% water (Karl Fischer)|
|≤5 ppm Heavy metals (as Pb)|
|pH||6.70 (37 °C, 0.1 M)(lit.)|
|7.00 (25 °C, 0.1 M)(lit.)|
|7.55 (5 °C, 0.1 M)(lit.)|
|useful pH range||7.0 - 9.0|
|suitability||cell culture tested|
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.
In convenient foil pouches
Trizma solutions can be autoclaved.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26|
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