|Related Categories||BioReagent Buffers, Biological Buffers, Buffer Convenience Packaging, Buffers A to Z, Good’s Buffers,|
|grade||Biotechnology Performance Certified|
|mol wt||average Mw 154.8|
|impurities||DNase, RNase, Protease, NICKase, none detected|
|total aerobic microbial count, tested|
|≤0.5% water (Karl Fischer)|
|≤5 ppm Heavy metals (as Pb)|
|pH||6.70 (37 °C, 0.05 M)(lit.)|
|7.00 (25 °C, 0.05 M)(lit.)|
|7.55 (5 °C, 0.05 M)(lit.)|
|useful pH range||7.0 - 9.0|
|suitability||cell culture tested|
Pre-mixed combinations of TRIZMA Base and TRIZMA HCl to provide the most common pH values for Tris buffers. Dissolve in water and use, no pH adjustment is necessary. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. Guaranteed accuracy: ±0.1 pH units. If a different concentration is desired, note the concentration effect: a 0.5 M solution will have a pH 0.05 units higher; a 0.005 M solution will have a pH 0.05 units lower. If the buffer is in a pre-weighed foil pouch, dissolve one packet in 900 mL of water; add additional water to 1 liter to make a 100 mM solution. Temperature: 25 °C.
Certain pH electrodes do not give accurate results with Tris buffers.
In convenient foil pouches
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26|
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