Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed.

General description

Regarded as the "Bible" of mammalian embryo manipulation techniques, the third edition has been completely reorganized and rewritten. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques.

Table of Contents

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future
Chapter 2. Summary of Mouse Development
Chapter 3. Production of Transgenic and Chimeric Mice: General Issues
Chapter 4. Recovery and In Vitro Culture of Pre-implantation–Stage Embryos
Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos
Chapter 6. Surgical Procedures
Chapter 7. Production of Transgenic Mice
Chapter 8. Isolation, Culture, and Manipulation of Blastocyst–derived Stem Cells
Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations
Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells
Chapter 11. Production of Chimeras
Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences
Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning
Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection
Chapter 15. Cryopreservation, Rederivation, and Transport of Mice
Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems
Chapter 17. Setting up and Equipping a Micromanipulation Lab
Appendices