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  • A0162 - Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

A0162 Sigma

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution



Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mouse
application(s)   direct ELISA: 1:3,000 using IgG, IgA, IgM
  western blot: 1:50 dilution
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C


Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.


Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect antibodies from hybridomas by indirect ELISA. The antibody was incubated in microtiter plates for 1 hour at 37°C and detected using p-nitrophenyl phosphate substrate (Sigma). Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect the reactivity of fungal cytoplasmic protein with proteins from the serum of mice infected with yeast-form Y cells by western blot analysis. The antibody was used at a 1:50 dilution.

General description

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells.

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Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


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Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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