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  • A0168 - Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

A0168 Sigma

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody



Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Antibody-Peroxidase Conjugates, MO-MR,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
species reactivity   mouse
application(s)   direct ELISA: 1:50,000
  immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
  western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C



purified mouse IgG Fc fragment

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal.


Peroxidase-conjugated goat anti-mouse IgG (Fc specific) antibody was used to detect the level of IgGs in mouse sera following Sv40Tag infection by indirect ELISA at a 1:1000 dilution in 10% normal goat serum for 30 minutes at 37°C. Peroxidase-conjugated goat anti-mouse IgG (Fc specific) antibody was used to detect the Sv40Tag mAB in murine breast tumor cell line 4T1 and kidney tumor cell line mKSA by western blot at a dilution of 1:2000 for 2 hours at room temperature. Western blot was developed using 3, 3′ diamino-benzidine reagent (Sigma) as the substrate.

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.
Specificity and cross-reactivity of anti-Mouse IgG (Fc Specific)-Peroxidase has been evaluated by ELISA. The conjugate is specific for mouse IgG and mouse IgG Fc fragment and shows no reactivity with mouse Fab fragment or human IgG. Furthermore, the antibody conjugates does not bind other mouse Igs.

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