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A0185 Sigma

Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse

clone BN-34, purified immunoglobulin

Synonym: Monoclonal Anti-Biotin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Biotin, B-BI, Biotin and Biotin Antibodies,
biological source   mouse
antibody form   purified immunoglobulin
clone   BN-34, monoclonal
packaging   vial of 0.5 mL
application(s)   dot blot: suitable
  flow cytometry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
  indirect ELISA: 1:30,000
  western blot (chemiluminescent): 1:80,000
isotype   IgG1
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

Immunogen

Biotin-KLH conjugate

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Monoclonal Anti-Biotin-Peroxidase antibody has been used for chemiluminescent western blot (1:200, 1:4000, 1:80,000), dot blot (1:4000), indirect ELISA (1:30,000) and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:300) applications. Additionally, the antibody may be used in amplification techniques, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

General description

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

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Protocols & Articles

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Antibody Basics

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