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A0224 Sigma

Anti-l/s-Afadin antibody produced in rabbit

affinity isolated antibody

Properties

Related Categories AE-AK, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins,
species reactivity   canine, rat, mouse, human
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
  indirect immunofluorescence: 1:250, using Cultured dog MDCK cells and cultured human HepG2 cells.
  microarray: suitable
  western blot: 1:1000 using extract of rat brain.
clone   polyclonal
antibody form   affinity isolated antibody
mol wt   antigen mol wt ~200 kDa (and 190 kDa)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to rat l/s afadin sequence, amino acids 1163-1179, with N-terminal cysteine added, conjugated to BSA.

General description

Afadin is a cell-cell adherens junction, F-actin binding multidomain protein. This adherens junction protein has two splicing variants: the ubiquitously expressed l-afadin, and the smaller s-afadin, which is abundantly expressed in neural tissues. Afadin associates with E-catenin proteins.
Anti-l/s-Afadin specifically binds to l-afadin and s-afadin by immunoblotting (approximately 200 and 190 kDa respectively). Additional bands may appear in certain extract preparations in western blot assays. The immunizing peptide specifically inhibits the staining of the I/s-afadin bands. The antibody is specific for human, dog, rat, and mouse l/s-afadin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Application

Rabbit anti-l/s-Afadin antibody has been used for immunohistochemistry (frozen sections, 1:1,000 using mouse liver sections) and indirect immunofluorescence (1:250 using cultured dog MDCK cells, and cultured human HepG2 cells) techniques. It can also be used for immunocytochemistry, microarray and western blot (1:1000 using rat brain extracts) assays.

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