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A0295 Sigma

Anti-Human IgA (α-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgA Secondary Antibodies and Conjugates, Peroxidase Labeled Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
species reactivity   human
application(s)   direct ELISA: 1:50,000
  dot blot: 1:80,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
  western blot: suitable
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified human IgA.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal as preservative

Application

Anti-human IgA (α-chain specific)-peroxidase antibody can be used in dot blot and western blotting.

Anti-human IgA (alpha-chain specific)-peroxidase antibody may also be used for immunohistochemistry using formalin-fixed, paraffin-embedded sections at a dilution of 1:100.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Peroxidase-conjugated goat anti-human IgA (alpha chain specific) was used in ELISA assays to detect antibodies to G. Lamblia in human saliva. Saliva was diluted in carbonate buffer and anti-human IgA was used at a concentration of 1:1,600. The antibody was detected using o-phenylenediamine dihydrochloride (Sigma) as a substrate.

General description

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines,,.
The antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA. Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material.
Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by ELISA. The conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.

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Peer-Reviewed Papers
15

References

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