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  • A0303 - Anti-Water Channel Aquaporin 3 antibody produced in rabbit

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A0303 Sigma

Anti-Water Channel Aquaporin 3 antibody produced in rabbit

affinity isolated antibody

Synonym: anti-AQP3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Aquaporin (Water) Channels, Antibodies to Ion Channels,
species reactivity   rat, mouse
application(s)   immunohistochemistry: suitable using Mouse kidney sections
  western blot: 1:200 using Rat kidney membranes
clone   polyclonal
antibody form   affinity isolated antibody
storage temp.   −20°C
Gene Information   mouse ... Aqp3(11828)
rat ... Aqp3(65133)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 275-292 of rat aquaporin 3 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde

General description

Aquaporins function in the movement of water and small solutes across the cell membrane. Aquaporins are widely distributed from bacteria to plants and animals. In E. coli, one aquaporin has been identified that is named AQP-Z. In the plant Arabidopsis thaliana, over 9 AQPs have been characterized. In mammals, at least 11 different AQPs have been cloned. AQP3 provides kidney medullary collecting ducts with high permeability to water, permitting water to move in the direction of an osmotic gradient and may play an important role in gastrointestinal tract water transport. AQP3 and AQP4 are located at the basolateral part of epithelial cells in kidney, colon and trachea.

Rabbit polyclonal anti-Water Channel Aquaporin 3 recognizes rat and mouse aquaporin 3 transport proteins.

Physical form

Lyophilized from a solution containing phosphate buffered saline, pH 7.4, 1% BSA, and 0.025% sodium azide

Preparation Note

Affinity isolated on immobilized Protein A

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Rabbit anti-AQP3 antibody was used in western blot analysis to identify the level of aquaporin 3 in E. solidaginis larvae. Anti-AQP3 was diluted 1:200 in 5% nonfat dry milk and incubated with the membrane for 2hrs at 21 degrees C.

Rabbit polyclonal anti-Water Channel Aquaporin 3 is used to tag aquaporin 3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aquaporin 3 in kidney medullary collecting ducts and the basolateral surfaces of epithelial cells involved in water transport.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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