• USA Home
  • A0310 - Monoclonal Anti-GABA antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
A0310 Sigma

Monoclonal Anti-GABA antibody produced in mouse

clone GB-69, ascites fluid

Synonym: Anti-γ-Aminobutyric acid

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Neurotransmission,
biological source   mouse
antibody form   ascites fluid
clone   GB-69, monoclonal
contains   15 mM sodium azide
species reactivity   wide range
application(s)   dot blot: 1:100
  immunofluorescence: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

GABA (γ-aminobutyric acid) conjugated to BSA

Physical form

Supplied as ascites fluid with 15 mM sodium azide as preservative.

Application

Gabaergic cells were identified in the rostal ventrolateral medulla of male Wistar rats by immunofluorescence with mouse monoclonal anti-GABA antibody and colocalized to the angiotensin II type 1 receptor. Sections were incubated in 2% BSA and 0.2% Triton X-100 in 0.01 M PBS for 30 min at 37°C to eliminate nonspecific staining.

Mouse monoclonal anti-GABA IgG can be used for immunohistochemistry, dot blot (1:100) and indirect ELISA.

General description

γ-Aminobutyric acid (GABA) functions as an inhibitory neurotransmitter in the central nervous system. It also functions as a neuromodulator in some peripheral tissues. Additionally, GABA is present in non-nervous tissues and regulates blood pressure, heart rate, respiration, immunomodulation, cell proliferation, protein synthesis and metabolism.
Monoclonal Anti-GABA (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The antibody does not cross react with L-α-aminobutyric acid, L-glutamic acid, L-aspartic acid, glycine, δ-aminovaleric acid, L-threonine, L-glutamine, taurine, putrescine, L-alanine and carnosine. However, weak cross-reaction is observed with β-alanine and ε-aminocaproic acid.

Price and Availability


Antibody Explorer
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?