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  • A0349 - Anti-l-Afadin antibody produced in rabbit

A0349 Sigma

Anti-l-Afadin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution



Related Categories AE-AK, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins,
species reactivity   human, canine, mouse, rat
application(s)   immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
  indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.
  microarray: suitable
  western blot: 1:2,000 using extract of rat brain
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~200 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)
biological source   rabbit
conjugate   unconjugated



Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

General description

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein , . AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality ,
Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide


Rabbit anti-l-afadin antibody is also suitable for use in microarray and indirect immunofluorescence assays.

Rabbit anti-l-afadin antibody may be used for immunocytochemistry on DLD1 cells, immunoblotting and also for immunohistochemistry .

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RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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