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A0409 Sigma

Anti-α1-Antitrypsin antibody produced in rabbit

IgG fraction of antiserum

Synonym: anti-A1AT

Properties

Related Categories AM-AN, Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, Primary Antibodies,
species reactivity   human
application(s)   immunoelectrophoresis: suitable
  indirect ELISA: 1:30,000-1:60,000
clone   polyclonal
antibody form   IgG fraction of antiserum
mol wt   antigen mol wt ~50 kDa
storage temp.   2-8°C
Gene Information   human ... SERPINA1(5265)
biological source   rabbit
packaging   vial of 2 mL lyophilized antiserum
conjugate   unconjugated

Description

Immunogen

Purified human α-1-antitrypsin

General description

α-1-antitrypsin belongs to the serpin family and is a strong serine protease inhibitor. Its pivotal role is to protect lower respiratory tract against proteolytic destruction by human leukocyte elastase. Mutation in AAT gene will reduce the serum concentration of α-1-antitrypsin and hence increase the risk of emphysema. Anti-α1-antitrypsin antibody (diluted 1:9000) can be used as a capture antibody for determination of transgene expressions levels. It can also be used in immunoelectrophoresis. Rabbit anti-α1-antitrypsin antibody reacts specifically with α1-antitrypsin of human.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Application

Anti-α1-antitrypsin antibody (diluted 1: 1000) can be used in ELISA. It can also be used for probing immunoblots to identify A1AT. Additionally, it can be used in Ouchterlony double diffusion.

Rabbit anti-α1-antitrypsin antibody can also be used in immunoelectrophoretic techniques.

Price and Availability


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