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  • A0409 - Anti-α1-Antitrypsin antibody produced in rabbit

A0409 Sigma

Anti-α1-Antitrypsin antibody produced in rabbit

IgG fraction of antiserum

Synonym: anti-A1AT



Related Categories AM-AN, Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, Primary Antibodies,
species reactivity   human
application(s)   immunoelectrophoresis: suitable
  indirect ELISA: 1:30,000-1:60,000
clone   polyclonal
antibody form   IgG fraction of antiserum
mol wt   antigen mol wt ~50 kDa
storage temp.   2-8°C
Gene Information   human ... SERPINA1(5265)
biological source   rabbit
packaging   vial of 2 mL lyophilized antiserum
conjugate   unconjugated



Purified human α-1-antitrypsin

General description

α-1-antitrypsin belongs to the serpin family and is a strong serine protease inhibitor. Its pivotal role is to protect lower respiratory tract against proteolytic destruction by human leukocyte elastase. Mutation in AAT gene will reduce the serum concentration of α-1-antitrypsin and hence increase the risk of emphysema. Anti-α1-antitrypsin antibody (diluted 1:9000) can be used as a capture antibody for determination of transgene expressions levels. It can also be used in immunoelectrophoresis. Rabbit anti-α1-antitrypsin antibody reacts specifically with α1-antitrypsin of human.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.


Anti-α1-antitrypsin antibody (diluted 1: 1000) can be used in ELISA. It can also be used for probing immunoblots to identify A1AT. Additionally, it can be used in Ouchterlony double diffusion.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-α1-antitrypsin antibody can also be used in immunoelectrophoretic techniques.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers


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