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A0433 Sigma

Anti-Albumin antibody produced in rabbit

IgG fraction of antiserum

Properties

Related Categories AL-AL, Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, Primary Antibodies,
species reactivity   human
application(s)   indirect ELISA: 1:25,000
  quantitative precipitin assay: 4.0-6.0 mg/mL
clone   polyclonal
antibody form   IgG fraction of antiserum
mol wt   antigen mol wt 65 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ALB(213)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

purified human albumin

General description

Albumin, the major protein produced by liver cells, represents more than half of the total protein content of human serum. Many other body fluids also contain albumin. Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.

Rabbit polyclonal anti-Albumin antibody reacts with human serum and human albumin. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum result in single arcs of precipitation in the γ region.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Application

Anti-Albumin antibody can be used for indirect ELISA (1:25,000) and quantitative precipitin assay (4-6 mg/ml).

Immunofluorescence on prostate cancer cells, PC3wtAR, was performed using the rabbit anti-albumin antibody. Prior to incubation with anti-albumin (1:200) the cells were fixed in methanol and blocked with 10% whole goat serum.

Rabbit polyclonal anti-Albumin antibody is used to tag albumin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of albumin a variety of clinical indications.

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