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  • A0545 - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

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A0545 Sigma

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Antibody-Peroxidase Conjugates, Molecular Biology,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
species reactivity   rabbit
does not react with   human
application(s)   direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
  western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)
conjugate   peroxidase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

purified rabbit IgG

Preparation Note

Prepared by two-step glutaraldehyde method.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.

Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

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BioFiles 2008, 3.6, 10.
Keywords: Biofiles, Immunofluorescence, PAGE, Western blot

Antibody Basics

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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
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Western Blot Tested Antibodies

During Western blot analysis, researchers report that failure most often occurs when trying to achieve successful antibody detection of target proteins. We know that this step is vital to experimenta...
Keywords: PAGE, Western blot

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Peer-Reviewed Papers
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