A0545 Sigma

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Antibody-Peroxidase Conjugates, Peroxidase Conjugates,
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
  western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

Antibody adsorbed with human IgG.

Preparation Note

Adsorbed to reduce background staining with human samples.

Prepared by two-step glutaraldehyde method2.

Specificity

Binds all rabbit Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Application

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.1

Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution. Peroxidase-conjugated goat anti-rabbit IgG antibody was used for wester blot analysis of yeast lysates following protein carbonylation assays at a dilution of 1:5000.

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