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A0561 Sigma

ACCUSPIN System-Histopaque®-1077

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Properties

Related Categories ACCUSPIN and Histopaque, Accuspin, Cell Biology, Density Gradient Media, General Stem Cell Biology,
sterility   aseptically filled
shelf life   Expiry date on the label.
packaging   package of 100 tubes
storage temp.   2-8°C

Description

Application

ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.

General description

γ-irradiated 50 mL polypropylene tubes fitted with a HDPE porous barrier and aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Protocols & Applications

Video Procedure for Accuspin

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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08168 Timestrip Plus 8 °C

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