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A0670 Sigma

Anti-Adenosine 3′,5′-Cyclic Monophosphate (cAMP) antibody produced in rabbit

whole antiserum

Synonym: Anti-cAMP

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Properties

Related Categories AD-AD, Alphabetical Index, Antibodies, Antibodies and Kits to Cyclic Nucleotides, Antibodies for Cell Biology,
biological source   rabbit
antibody form   whole antiserum
clone   polyclonal
species reactivity   wide range
application(s)   immunohistochemistry: suitable
  radioimmunoassay: 1:10
conjugate   unconjugated
storage temp.   2-8°C

Description

Immunogen

Cyclic AMP-2′-BSA

Reconstitution

Reconstitute with 1ml 0.1% BSA in distilled water. Store the reconstituted stock at -20°C. Working dilution (1:10) to be discarded if unused within 12 hours.

Physical form

The antibody is provided as a pre-diluted antiserum that has been lyophilized.

Application

Anti-Adenosine 3′,5′-Cyclic Monophosphate (cAMP) antibody is suitable for use in radioimmunoassays. It can also be used for immunohistochemical assays.

Rabbit anti-cAMP was used in an enzyme immunoassay to detect the level of cAMP in human CD34+ peripheral blood mononuclear cells. Anti-cAMP was incubated with cells on a microtiter plate at room temperature on a shaker (300 rpm) for 2 hours.

General description

3′5′-Cyclic Adenosine Monophosphate (c-AMP) is an intracellular hormonal mediator that facilitates the glycogenolytic effects of epinephrine and glucagon in the liver. c-AMP is also mediates the effect of several other hormones, and is known to function as a second messenger for intracellular signal transductions.
Anti-Adenosine 3′,5′-Cyclic Monophosphate (cAMP) is specific for cAMP and does not cross-react with 5′-AMP, 5′-ADP, 5′-ATP or 3′,5′-cGMP.

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