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A0793 Sigma

Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, DM-DP, Dog Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
species reactivity   dog
application(s)   direct ELISA: 1:6,000
  dot blot: 1:4,000
  western blot (chemiluminescent): 1:4,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

IgG isolated from pooled normal dog serum

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Application

Alkaline-phosphatase rabbit anti-dog IgG antibody was used in ELISA assays to detect IgGs to the antigen B. burgdorfer in canine sera samples at a dilution of 1:1000.

Anti-Dog IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in dot blot (1:4,000), direct ELISA (1:4,000) and in chemiluminescent western blot (1:4,000).

Rabbit anti-dog IgG (whole molecule)-alkaline phosphatase antibody has been used as a secondary antibody in ELISA and immunoblot assays for the screening of autoreactive antibodies in female dogs.

General description

Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs. Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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