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A0844 Sigma

Anti-AP-2α antibody produced in rabbit

affinity isolated antibody, PBS solution

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Properties

Related Categories AA - CA, AO-AQ, Alphabetical Index, Antibodies, Antibodies for Gene Regulation,
species reactivity   chicken, human, mouse
application(s)   indirect immunofluorescence: 1:100 using cultured human HeLa cells
  microarray: suitable
  western blot: 1:250 using a nuclear extract from HeLa human carcinoma cells
clone   polyclonal
antibody form   affinity isolated antibody
form   PBS solution
mol wt   antigen mol wt ~50 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... TFAP2A(7020)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to human AP-2α, C-terminal sequence, (amino acids 420-437) conjugated to KLH with glutaraldehyde.

General description

Activation Protein transcription factor 2 (AP-2) is a developmentally modulated transcription factor that binds to specific sequence such as CCCCAGGC or related sequences. These sequences are usually present in several promoter and enhancer gene regions, including the promoters of the AP-2 genes themselves,,. AP-2 expression has also been linked to human breast cancer cells.
By immunoblotting, rabbit anti-AP-2α reacts specifically with human AP-2α. The sequence recognized by the antibody differs from the corresponding mouse sequence by one residue and from the chicken sequence by 2 residues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Application

Rabbit anti-AP-2alpha antibody can be used for western blot (1:250), microarray and indirect immunofluorescence (1:100) assays.

Price and Availability


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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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