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A0847 Sigma

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein

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Properties

Related Categories Adenylyl Cyclase Activators, Cell Biology, Cell Signaling and Neuroscience, Cyclic Nucleotide Metabolism, G Proteins and Cyclic Nucleotides More...
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt 177 kDa by amino acid sequence
storage temp.   2-8°C
Gene Information   human ... CAMP(820)
mouse ... CAMP(12796)
rat ... CAMP(316010)

Description

Biochem/physiol Actions

Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.

Reconstitution

When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.

Unit Definition

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
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