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  • A0856 - Anti-ATG5 (N-terminal) antibody produced in rabbit

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A0856 Sigma

Anti-ATG5 (N-terminal) antibody produced in rabbit

affinity isolated antibody, PBS solution

Synonym: Anti-Apoptosis-specific protein, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Autophagy protein 5-like

Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology,
species reactivity   human, rat, mouse
application(s)   immunofluorescence: suitable
  western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   PBS solution
mol wt   antigen mol wt ~56 kDa (Atg5-Atg12 complex)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

General description

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation,. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties,. Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

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