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A0919 Sigma

Mouse IgG−Agarose

(Suspension in 0.5 M NaCl containing 0.01% thimerosal)

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Properties

Related Categories Adsorption Resins, Antibodies, Antibody Purification and Characterization, Supplementary Products
antibody form   purified immunoglobulin
form   (Suspension in 0.5 M NaCl containing 0.01% thimerosal)
extent of labeling   1 mg per mL
application(s)   immunoprecipitation: suitable
matrix   Cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
matrix spacer   1 atom
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Packaging

2, 5 mL in glass bottle

Physical form

Suspension in 0.5 M NaCl containing 0.01% thimerosal

Application

IgG-Agarose can be used as an immunoadsorbent. It can also be used to affinity purify antibodies, remove species specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgGAgarose (resin volume). Mouse IgG-Agarose has been used for several immunoprecipitation assays,.

Mouse IgGs conjugated to agarose beads were used in immunoprecipitation assays to pre-clear HEK 293 whole cell extracts. Beads were incubated with extracts for 1 hour rotating at 4°C.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

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Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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