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A0924 Sigma

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 49:1 (ratio)

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Properties

Related Categories Acrylamide, Acrylamides, Molecular Biology, Nucleic Acid Electrophoresis, Protein Electrophoresis,
grade   for molecular biology
InChI Key   OCQZXMCGTAWGEQ-UHFFFAOYSA-N
product line   BioReagent
feed ratio   49:1 (ratio)
foreign activity   DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected

Description

Application

Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Packaging

1 L in poly bottle

100 mL in poly bottle

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Biochem/physiol Actions

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Price and Availability


Amicon Pro Purification System
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 2074 6.1 / PGIII
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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