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  • A1043 - Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

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A1043 Sigma

Anti-Chicken IgY (IgG) (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alkaline Phosphatase, Alphabetical Index, Antibodies, CE-CH, Chicken Secondary Antibodies and Conjugates,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:15,000
  dot blot: 1:60,000-1:80,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
  western blot (chemiluminescent): 1:80,000
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, and 15 mM sodium azide.

Application

Rabbit anti-chicken IgG alkaline phosphatase conjugated was used for western blot analysis of chicken protein lysates at a dilution of 1:1000 for 1 hour shaking at room temperature. Rabbit anti-chicken IgG alkaline phosphatase conjugated was used for western blot analysis of crude supernatant from A. castellanii trophozoites cultures at a 1:2000 dilution for 1 hour in 5% nofat dry milk.

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