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A1052 Sigma

Anti-Gliadin (Wheat)–Peroxidase Conjugate antibody produced in rabbit

fractionated antiserum, PBS solution

Synonym: Anti-Gliadin (Wheat)

Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Miscellaneous, Plant Biology,
species reactivity   barley, oats, rye, soy
  wheat
does not react with   potato, rice
application(s)   direct ELISA: 1:1,000
  dot blot: 1:500
  immunoblotting: suitable
clone   polyclonal
antibody form   fractionated antiserum
form   PBS solution
shipped in   dry ice
storage temp.   −20°C
biological source   rabbit
conjugate   peroxidase conjugate

Description

Immunogen

native and heat-treated wheat gliadin

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Application

Rabbit anti-gliadin (wheat)-peroxidase conjugate antibody can be used for immunoblot and ELISA(1:1000).

Biochem/physiol Actions

Gliadin is a class of glutamine containing, alcohol soluble proteins separable from wheat and rye glutens known as the prolamins. These proteins are associated with the harmful effects of celiac disease and gluten sensitive enteropathy in humans, causing characteristic changes in the small intestinal mucosa. In such cases, strict compliance to a gluten-free diet is usually recommended.

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