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A1054 Sigma

Monoclonal Anti-Digoxin–Alkaline Phosphatase antibody produced in mouse

clone DI-22, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Digoxin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Digoxin, DE-DL, Molecular Biology,
biological source   mouse
recombinant   expressed in mouse cell line
antibody form   purified immunoglobulin
clone   DI-22, monoclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate
  direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate
  dot blot: 1:20,000 using digoxigenin-labeled biomolecules
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

Digoxin-KLH.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide

Application

Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules).

Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets.

General description

Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin.

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
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