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A1357 Sigma

Anti-ACTR3 (236-250) antibody produced in rabbit

IgG fraction of antiserum, PBS solution

Synonym: Anti-ARP3, Anti-Actin-related protein 3 homolog

Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin,
species reactivity   human
application(s)   indirect immunofluorescence: suitable
  western blot: 1:500-1:1,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   PBS solution
mol wt   antigen mol wt ~47 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTR3(10096)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 236-250 of human ACTR3.

General description

Actin-related protein 3 (ARP3) exists within cells in a complex with ARP2. The ARP2/3 complex localizes to the leading edge of migrating cells and participates in nucleation of actin filaments. ARP3 is specifically involved in the nucleation process.

Rabbit anti-ACTR3 (236-250) antibody binds to human ACTR3 (236-250).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide.

Application

Rabbit anti-ACTR3 (236-250) antibody can be used for western blot (1:500-1:1,000) and indirect immunofluorescence.

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

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