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A1720 Sigma

G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Synonym: Antibiotic G418

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic powders, Antibiotics,
InChI Key   UHEPSJJJMTWUCP-NKCAIAFTSA-N
product line   BioReagent
form   powder
potency   ≥450 μg per mg (Dried basis)
solubility   H2O: soluble50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
storage temp.   2-8°C

Description

Caution

The product is stable for three years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Packaging

1, 5, 25 g in glass bottle

Preparation Note

G418 powder is soluble in water at 50 mg/mL.

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.

General description

G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Antibiotic Selection Guide

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Peer-Reviewed Papers
15

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