A1720 Sigma

G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Synonym: Antibiotic G418



Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic powders, Antibiotics,
product line   BioReagent
form   powder
optical activity   [α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
potency   ≥450 μg per mg (Dried basis)
color   white
solubility   H2O: soluble50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
storage temp.   2-8°C



The product is stable for three years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.


1, 5, 25 g in glass bottle

Preparation Note

G418 powder is soluble in water at 50 mg/mL.


G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.

General description

G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Protocols & Articles

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