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A1765 Sigma

S-Acetyl-coenzyme A synthetase from baker's yeast (S. cerevisiae)

lyophilized powder, ≥3 units/mg protein

Synonym: Acetate CoA ligase (AMP forming), Acetate thiokinase

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Properties

Related Categories 6.2.x.x C-S Bonds, 6.x.x.x Ligases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
composition   Protein, 10-30% biuret
storage temp.   −20°C

Description

Packaging

5 mg in poly bottle

Package size based on protein content.

Unit Definition

One unit will form 1.0 μmole of S-acetyl coenzyme A from acetate, ATP, and coenzyme A per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing stabilizers as potassium phosphate, sucrose, and reduced glutathione

Application

S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies.

Biochem/physiol Actions

Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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