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A1812 Sigma

Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-FITC

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Fluorescent Labels, Biochemicals and Reagents, FB-FJ,
biological source   mouse
recombinant   expressed in mouse cell line
antibody form   purified immunoglobulin
clone   FL-D6, monoclonal
form   buffered aqueous solution
application(s)   dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate
  indirect ELISA: 1:30,000
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Immunogen

FITC-BSA conjugate

Specificity

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide

Application

Alkaline phosphatase-conjugated mouse anti-FITC antibody was used for immunohistochemistry on mouse spleens. Tissue sections were fixed in acetone, blocked with PBS/5% goat serum/ 0.1% Tween and incubated with a FITC-conjugated primary antibody prior to the addition of the AP-conjugated anti-FITC antibody.

Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent such as alkaline phosphatase or horseradish peroxidase. Mouse monoclonal clone FL-D6 anti-FITC−Alkaline Phosphatase conjugate antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It can be used in ELISA and immunofluorescent techniques.

General description

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten.

Mouse monoclonal clone FL-D6 anti-FITC−Alkaline Phosphatase conjugate antibody reacts with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate). Monoclonal Anti-FITC recognizes the free FITC molecule and FITC conjugated to various biomolecules such as proteins (immunoglobulins, enzymes), oligonucleotides, nucleic acids and other ligands.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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