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A1933 Sigma

Bovine Serum Albumin

chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Albumins, Apoptosis and Cell Cycle, Biochemicals and Reagents, Bovine Serum Albumins,
product line   BioReagent
assay   ≥98%
form   lyophilized powder
mol wt   mol wt ~66 kDa
purified by   chromatography
packaging   poly bottle of
origin   New Zealand origin
impurities   ≤1 EU/mg endotoxin
pH   7
solubility   water: soluble (40 mg/ml)
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Packaging

1, 5, 25, 100 g in poly bottle

Preparation Note

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Peer-Reviewed Papers
15

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