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  • A1949 - Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

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A1949 Sigma

Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

Synonym: Monoclonal Anti-Rabbit IgG (γ-chain specific)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibody Conjugates, Molecular Biology, Peroxidase Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   RG-96, monoclonal
form   lyophilized powder
species reactivity   rabbit
does not react with   pig, rat, human, bovine, horse, feline, chicken, guinea pig, goat, canine, sheep
packaging   vial of 0.5 mL
application(s)   direct ELISA: 1:40,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
  western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
isotype   IgG1
conjugate   peroxidase conjugate
storage temp.   2-8°C

Description

Immunogen

Rabbit IgG

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal.

Application

Monoclonal Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody can be used in chemiluminescent western blot at 1:10,000 dilutions in human gastric and pancreatic carcinoma cell lines and immunohistochemistry (at 1:200 dilutions in formalin-fixed, paraffin-embedded sections).

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

General description

Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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