A1978 Sigma

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-15, purified immunoglobulin, buffered aqueous solution

DOWNLOAD MSDS (PDF)

Synonym: Monoclonal Anti-β-Actin

Properties

Related Categories A, AC-AC, Actin Antibodies, Alphabetical Index, Antibodies,
species reactivity   canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine
does not react with   Dictyostelium discoideum
application(s)   immunocytochemistry: 10-40 μg/mL using human foreskin fibroblasts
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: suitable
  microarray: suitable
  western blot: 0.5-1 μg/mL using cell extract of human foreskin fibroblasts or chicken fibroblasts.
clone   AC-15, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 42 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Description

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Application

The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

Monoclonal mouse anti-actin antibody was uses as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.1

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Price and Availability

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